Comparative Data Practice

PFU_OROV

Weishaupt A-K, Lamann K, Tallarek E, Pezacki AT, Matier CD, Schwerdtle T, Aschner M, Chang CJ, Stürzenbaum SR and Bornhorst J (2024) Dysfunction in atox-1 and ceruloplasmin alters labile Cu levels and consequently Cu homeostasis in C. elegans. Front. Mol. Biosci. 

cytokine setups

Arra A, Lingel H, Pierau M and Brunner-Weinzierl MC (2023) PD-1 limits differentiation and plasticity of Tc17 cells. Front. Immunol.

p21 GAPDH

Guo X, Xu K, Wang L, Ding L, Li W, Zhang X, Zhao W, Wang N, Wang G, Zhao W, Rosas I and Yu G (2024) Triiodothyronine acts on DAO to regulate pulmonary fibrosis progression by facilitating cell senescence through the p53/p21 signaling pathway. Front. Pharmacol. 

In an experiment in which FCCP alone was administered to healthy mammalian cells in order to determine whether the protonophore can induce loss of mitochondrial membrane potential (ΔΨm) and trigger apoptotic signaling, what would be the appropriate control group?

MAPK enhances ERK phosphorylation

PI3K in the absence of PIP3 has no effect on apoptosis

AMPK activation enhances mitochondrial biogenesis in muscle cells under hypoxic conditions.

Phosphorylation of CREB increases as intracellular cAMP levels rise in neuronal cells.

Pro-inflammatory cytokine release is elevated in C57BL/6 cells exposed to lipopolysaccharides, under low glucose concentrations.

To test whether forced overexpression of TRF2 — a shelterin complex protein — is sufficient to prevent stress-induced premature senescence (SIPS) in human fibroblasts exposed to sublethal oxidative stress, what is the most appropriate control condition?