Concept Check: DERK Amino Acids
Concept Check: DERK Amino Acids
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Question 1 of 3
1. Question
An anion exchange column, which binds negatively-charged molecules, is frequently used to isolate proteins with differing charges. A collection of different proteins are poured into the column setup and those with negative charges will stick to the column while neutral or positively charged molecules are washed away. Given this information which of the following peptide sequences will bind MOST strongly to the column?
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Question 2 of 3
2. Question
A cation exchange column is identical to an anion exchange column except it binds positively charged molecules rather than negative ones. Given this information which of the following peptide sequences will bind with the second-highest affinity to a cation exchange column?
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Question 3 of 3
3. Question
At physiological pH, the amino acid sequence, LTYEFDKG, has a net charge of:
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Remember DERK are the only charged amino acids.