Concept Check: Protein Gel Electrophoresis

An E6V mutation in the beta subunit of hemoglobin leads to the fatal disease sickle cell anemia. Scientists trying to isolate hemoglobin using isoelectric focusing from sickle cell anemia patients would expect to find the mutated hemoglobin:

Closer to the basic side of the gel as compared to a WT sample
Closer to the acidic side of the gel as compared to the WT sample
At the same location as the WT sample
Possess a darker band as compared to WT

If a homotrimeric protein is composed of 210 amino acid residue monomers at which kDa marker would you find this protein when run via Native PAGE?

11.6 kDa
23.1 kDa
46.2 kDa
69.3 kDa

If a homodimer of heterodimers is held together by ionic interactions between its large homodimer subunits and disulfide bridges between its smaller heterodimers within the subunits it would show ___ bands on an SDS gel and ___ bands on an SDS + reducing gel?

2,4
4,2
1,2
2,1