Experiment Terminology

Experiment Terminology

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Question 1 of 6

1. Question
What does the notation Nf1-/- signify in the context of genetics?
- Overexpression of the Nf1 gene
- One functional copy and one non-functional copy of the Nf1 gene
- A mutation in one copy of the Nf1 gene
- The absence of the Nf1 gene in an organism
Question 2 of 6

2. Question
Which technique is commonly used to create a cell line or organism with a specific gene completely inactivated?
- PCR Amplification
- Gene Knockout
- Vector Transformation
- Plasmid Introduction
Question 3 of 6

3. Question
An Rb +/- cell has:
- one dominant and one recessive allele of the Rb gene.
- an Rb gene that has undergone amplification.
- two functional Rb alleles.
- no functional Rb alleles.
Question 4 of 6

4. Question
Which of the following best describes an empty vector?
- A vector that contains a full set of genes from an organism
- A vector that has lost its ability to replicate
- A vector that does not contain any insert DNA or gene of interest
- A vector that is capable of expressing proteins without any modifications
Question 5 of 6

5. Question
A WT cell with a FOXO1 vector transfection would:
- have the same levels of FOXO1 as a non-transfected WT cell
- have higher levels of FOXO1 as a non-transfected WT cell
- have lower levels of FOXO1 as a non-transfected WT cell
Question 6 of 6

6. Question
Why might a scientist use an empty vector in an experiment?
- To serve as a control to distinguish between the effects of the vector backbone and the inserted gene
- To increase the likelihood of gene deletion in host cells
- Because empty vectors are more cost-effective than vectors with inserts
- To enhance the expression of native genes in the host organism

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