Blotting

GLUT 4 is a 509 amino acid insulin-regulated glucose transporter found in adipose tissue and striated muscle. A variety of different isoforms of the GLUT4 also exist including GLUT 4Δ3-5 which lacks exons 3-5 and only contains 371 amino acids.

To determine whether different cardiac muscle cell lines express different amounts of each isoform scientists isolated both GLUT 4 and GLUT 4Δ3-5 from three separate cell lines and visualized the amount of each glucose transporter using a Western blot (Figure 1.)

Figure 1: Western blot of cardiac muscle cell lines 

Which of the following is supported by the Western blot?

Which technique could be used to measure the amount of complement component 3 transcripts present in the plasma of different mouse lines?

Facioscapulohumeral muscular dystrophy (FSMD) is caused by deletion of DNA near the DUX4 gene. Scientists can probe for the FSMD deletion using an EcoRI/Avrll double digestion and subsequent visualization of the digested gene products using which of the following techniques? 

O-GalNAc glycans are attached to the hydroxyl group of serine and threonine residues in the Golgi apparatus. Following glycosylation, these proteins are transported to the cell’s surface where they mediate a wide variety of receptor ligand interactions. Which of the following techniques could be used to measure the changes in O-GalNAc glycosylation in cancer cells?